Bisphenol A (BPA) was used in the plastic industry and widely distributed in the environment, which might cause adverse effects to living organisms. In order to study the effects of BPA on the organs of mice brain, liver and testical, the KM mice were administrated to different concentrations of BPA (0, 0.09, 0.9, 9 mg/kg·d). After 14 days, the reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH concentrations in the three organs were measured. Results showed that the ROS and MDA contents were increased with the added concentration of the BPA. But the GSH content was decreased. It is indicated BPA can induce the mice’s oxidative damage and the effect is much easier for the brain.